2021-06-27 20:16 Fluorescence-based determination is becoming more and more important in the biophysical study of the structure, function and kinetics of nucleic acids. The emergence of single molecule fluorescence spectroscopy provides insight into many of the dynamic and mechanical details previously hidden by integrated techniques. These studies require the stable integration of fluorescent tags into the target system at a specific location. Ribonucleic acid (RNA) and deoxyribonucleic acid (DNA) do not contain natural fluorescent groups. Therefore, in order to carry out fluorescence determination, it is necessary to introduce these groups. Fluorescence labeling of nucleic acids is usually carried out by enzymatic reaction. Organic fluorescent group chemistry was introduced into primers or nucleosides triphosphate, and then amplified by PCR or incorporated into DNA or RNA polymerase or terminal polynucleotides transferase.
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