2020-12-21 20:40 Whole genome sequencing (WGS) has the capacity to greatly enhance genomic knowledge and understand mysteries of life by utilizing the most advanced genetic sequencing technologies. WGS can be used for variant calling, genome annotation, phylogenetic analysis, reference genome construction, and more. WGS tries to cover the whole genome, but actually covers 95% of the genome with technical difficulties in sequencing regions such as centromeres and telomeres. Another challenge for WGS is data management. As larger datasets become more accessible and affordable, computational analysis will be the rate-limiting factor rather than sequencing technology. Here we will discuss the bioinformatics workflow for detection of genetic variations in WGS to help you get through it.
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